Polypeptide is a kind of compound formed by amino acid residues linked by peptide bond, which has a wide range of biological activities. Some sweeteners, toxins, antibiotics are also polypeptide compounds. There are four steps in the synthesis of peptides: 1. Protecting non reactive groups, 2. Activating carboxyl groups, 3. Forming peptide bonds, 4. Deprotecting groups.

In the chemical synthesis of polypeptide, especially in the activation step, there is the possibility of amino acid racemization. In addition, there are many sequence like polypeptides in the final product of chemical synthesis, so it is difficult to separate and purify the product.

The best way to solve these problems is to simulate organism and use heteroenzyme to catalyze the synthesis of peptide.

In the past decade, enzyme catalyzed peptide synthesis has been widely studied, developed and applied. Compared with chemical method, it has obvious advantages over chemical method for environmental protection and human health.

In the past, peptide drugs were chemically synthesized or expressed by genes. The amount of organic solvents used in chemical synthesis is large, most of them are toxic organic solvents, which are harmful to human body and environment. Gene expression method often needs mammalian expression system to obtain recombinant peptide with biological active peptide. This method has large investment, high production cost and is not easy to industrialize.

For the synthesis of small folding peptides, enzymatic synthesis by protease is a good alternative method. The method of enzymatic synthesis by protease is a site-specific and stereospecific method, which can avoid the effect of spinning, and it is a reaction in mild and non-aqueous phase, which can reduce the protection of side chain.

Compared with the mature step-by-step chemical synthesis method, enzyme method is more competitive in the following aspects: first, the synthesis of short peptides with existing free functional side chains, such as Tiandong aminophenylalanine methyl ester; second, the semi synthesis of peptides and proteins, such as insulin; third, the condensation of unprotected peptide fragments synthesized by solid-phase synthesis; fourth, the post modification or labeling obtained by recombinant DNA technology The C-terminal part of the peptide.

The advantages of enzymatic method over chemical method are: high selectivity of main body; high selectivity of region; narrow range of amino acids; low requirements of group protection; medium purity of substrate; no by-product; no racemization.

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About Shengnuo

Chengdu Shengnuo Biotechnology Co., Ltd. has "Chengdu polypeptide drug engineering technology research center" in Chengdu, mainly engaged in polypeptide, polypeptide drug and beauty peptide research. Our zero defect has passed the FDA certification, and now it has become the first-class professional peptide drug and product development, technology transfer, technical service and peptide drug industry in the scale production and export of China's parks.